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验证数据展示

  • FC experiment of SW 1990 using CL488-67886

    FC experiment of SW 1990 using CL488-67886

    1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-HUMAN KDM1B (CL488-67886, Clone:1E1A3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated KDM1B Monoclonal antibody

KDM1B Monoclonal Antibody for FC (Intra)
Cat No. CL488-67886

产品说明书

CloneNo. 1E1A3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat, mouse

应用

FC (Intra)

别名

AOF1, LSD2, C6orf193, 1E1A3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inSW 1990 cells
For other applications, we recommend the unconjugated version of this antibody, 67886-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67886 targets KDM1B in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat, mouse
免疫原

CatNo: Ag30672

Product name: Recombinant human KDM1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 666-822 aa of NM_001364614

Sequence: FPYRFWDSKVQGADFFGHVPPSASKRGLFAVFYDMDPQKKHSVLMSVIAGEAVASVRTLDDKQVLQQCMATLRELFKEQEVPDPTKYFVTRWSTDPWIQMAYSFVKTGGSGEAYDIIAEDIQGTVFFAGEATNRHFPQTVTGAYLSGVREASKIAAF

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 amine oxidase (flavin containing) domain 1
别名 AOF1, LSD2, C6orf193, 1E1A3
计算分子量 92 kDa
观测分子量 92 kDa
GenBank蛋白编号NM_001364614
基因名称 KDM1B
Gene ID (NCBI) 221656
RRIDAB_2923817
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NB78
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 KDM1B antibody CL488-67886Download protocol
Standard Protocols
Click here to view our Standard Protocols