验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, K-562 cells, C6 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells |
| Positive FC (Intra) detected in | SW 1990 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67886-1-Ig targets KDM1B in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag30672 Product name: Recombinant human KDM1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 666-822 aa of NM_001364614 Sequence: FPYRFWDSKVQGADFFGHVPPSASKRGLFAVFYDMDPQKKHSVLMSVIAGEAVASVRTLDDKQVLQQCMATLRELFKEQEVPDPTKYFVTRWSTDPWIQMAYSFVKTGGSGEAYDIIAEDIQGTVFFAGEATNRHFPQTVTGAYLSGVREASKIAAF 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | amine oxidase (flavin containing) domain 1 |
| 别名 | AOF1, LSD2, C6orf193, 1E1A3 |
| 计算分子量 | 92 kDa |
| 观测分子量 | 92 kDa |
| GenBank蛋白编号 | NM_001364614 |
| 基因名称 | KDM1B |
| Gene ID (NCBI) | 221656 |
| RRID | AB_2918643 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8NB78 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM1B antibody 67886-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
