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验证数据展示

  • WB analysis using 68613-1-Ig

    WB analysis using 68613-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68613-1-Ig (KANK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68613-1-Ig

    IF Staining of HepG2 using 68613-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KANK1 antibody (68613-1-Ig, Clone: 2D3G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of HepG2 using 68613-1-Ig

    FC experiment of HepG2 using 68613-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KANK1 Monoclonal antibody (68613-1-Ig, Clone:2D3G8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

KANK1 Monoclonal antibody

KANK1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68613-1-Ig

产品说明书

CloneNo. 2D3G8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

ANKRD15, Ankyrin repeat domain-containing protein 15, KANK, KIAA0172, Kidney ankyrin repeat-containing protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, PC-3 cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, HuH-7 cells, Daudi cells, Raji cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KANK1 Ready-To-Use IHC Kit. KANK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68613-1-Ig targets KANK1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31440

Product name: Recombinant human KANK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 291-467 aa of NM_015158

Sequence: ISVLQEEKRQLVSQLKNQRAASQINVCGVRKRSYSAGNASQLEQLSRARRSGGELYIDYEEEEMETVEQSTQRIKEFRQLTADMQALEQKIQDSSCEASSELRENGECRSVAVGAEENMNDIVVYHRGSRSCKDAAVGTLVEMRNCGVSVTEAMLGVMTEADKEIELQQQTIESLKE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KN motif and ankyrin repeat domains 1
别名 ANKRD15, Ankyrin repeat domain-containing protein 15, KANK, KIAA0172, Kidney ankyrin repeat-containing protein
计算分子量147KD
观测分子量130-200 kDa
GenBank蛋白编号NM_015158
基因名称 KANK1
Gene ID (NCBI) 23189
RRIDAB_3085307
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KANK1 (KN motif and ankyrin repeat domain-containing protein 1) is involved in the control of cytoskeleton formation by regulating actin polymerization, inhibiting actin fiber formation and cell migration (PMID: 25961457). It inhibits RhoA activity, the formation of lamellipodia but not of filopodia (PMID: 25961457, PMID: 5961457).It also inhibits fibronectin-mediated cell spreading and regulates Rac signaling pathways (PMID: 25961457). Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions (PMID: 27410476). KANK1 can be detected 130-200 kDa (PMID: 15823577, PMID: 33253712, PMID: 35805114).

实验方案

Product Specific Protocols
IF protocol for KANK1 antibody 68613-1-IgDownload protocol
WB protocol for KANK1 antibody 68613-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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