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验证数据展示

  • WB analysis using CL488-66848

    WB analysis using CL488-66848

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66848 (JTV1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-66848

    FC experiment of HeLa using CL488-66848

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated JTV1 Monoclonal antibody (CL488-66848, Clone:1D6B5)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated JTV1 Monoclonal antibody

JTV1 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-66848

产品说明书

CloneNo. 1D6B5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

1D6B5, AIMP2, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, HLD17, P38

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AIMP2/JTV1 Ready-To-Use IHC Kit. AIMP2/JTV1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66848-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66848 targets JTV1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0675

Product name: Recombinant human JTV1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC002853

Sequence: MPMYQVKPYHGGGAPLRVELPTCMYRLPNVHGRSYGPAPGAGHVQEESNLSLQALESRQDDILKRLYELKAAVDGLSKMIQTPDADLDVTNIIQADEPTTLTTNALDLNSVLGKDYGALKDIVINANPASPPLSLLVLHRLLCEHFRVLSTVHTHSSVKSVPENLLKCFGEQNKKQPRQDYQLGFTLIWKNVPKTQMKFS

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 JTV1 gene
别名 1D6B5, AIMP2, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, HLD17, P38
计算分子量 36 kDa
观测分子量 32-36 kDa
GenBank蛋白编号BC002853
基因名称 JTV1
Gene ID (NCBI) 7965
RRIDAB_3672926
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13155
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 JTV1 antibody CL488-66848Download protocol
WB protocol for CL Plus 488 JTV1 antibody CL488-66848Download protocol
Standard Protocols
Click here to view our Standard Protocols
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