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CoraLite® Plus 488-conjugated JNK Recombinant antibody

JNK Recombinant Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6J16

Cat No : CL488-81629

Print datasheet

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

验证数据展示

  • IF Staining of Jurkat using CL488-81629

    IF Staining of Jurkat using CL488-81629

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 JNK antibody (CL488-81629, Clone: 6J16 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-81629

    FC experiment of HeLa using CL488-81629

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human JNK (CL488-81629, Clone:6J16) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inJurkat cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 81629-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81629 targets JNK in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen JNK fusion protein Ag1639 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase 8
Synonyms c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight44-48 kDa, 50-55 kDa
GenBank Accession NumberBC130572
Gene Symbol MAPK8
Gene ID (NCBI) 5599
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45983
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has some isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). This antibody can recognize JNK1, JNK2 and JNK3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 JNK antibody CL488-81629Download protocol
FC protocol for CL Plus 488 JNK antibody CL488-81629Download protocol
Standard Protocols
Click here to view our Standard Protocols