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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98328-3-RR

    FC experiment of human peripheral blood leukocytes using 98328-3-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human JAM-A/CD321 Rabbit RecAb (98328-3-RR, Clone: 242290B3) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98328-3-RR

    FC experiment of human peripheral blood leukocytes using 98328-3-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human JAM-A/CD321 Rabbit RecAb (98328-3-RR, Clone:242290B3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human JAM-A/CD321 Rabbit Recombinant Antibody

JAM-A/CD321 Uni-rAb® Recombinant Antibody for FC
Cat No. 98328-3-RR

产品说明书

CloneNo. 242290B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

F11R, JAM-A/Junctional Adhesion Molecule A, 242290B3, CD321, F11 receptor

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98328-3-RR targets JAM-A/CD321 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1372

Product name: Recombinant Human JAM-A/Junctional Adhesion Molecule A protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-238 aa of NM_016946.6

Sequence: SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 F11 receptor
别名 F11R, JAM-A/Junctional Adhesion Molecule A, 242290B3, CD321, F11 receptor
计算分子量33kDa
GenBank蛋白编号NM_016946.6
基因名称 F11R
Gene ID (NCBI) 50848
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y624-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Junctional adhesion molecule A (JAM-A), also known as CD321 or F11 receptor (F11R), was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.

实验方案

Product Specific Protocols
FC protocol for JAM-A/CD321 antibody 98328-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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