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JAK3 Recombinant antibody

JAK3 Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3N1

Cat No : 80331-1-RR

Print datasheet

Synonyms

JAK 3, JAK3, JAK3_HUMAN, JAKL, Janus kinase 3, L JAK, Leukocyte janus kinase, LJAK, Tyrosine protein kinase JAK3

验证数据展示

  • WB analysis using 80331-1-RR

    WB analysis using 80331-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80331-1-RR (JAK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 80331-1-RR

    IHC staining of human lymphoma using 80331-1-RR

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

80331-1-RR targets JAK3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Janus kinase 3 (a protein tyrosine kinase, leukocyte)
Synonyms JAK 3, JAK3, JAK3_HUMAN, JAKL, Janus kinase 3, L JAK, Leukocyte janus kinase, LJAK, Tyrosine protein kinase JAK3
Calculated Molecular Weight 1124 aa, 125 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC028068
Gene Symbol JAK3
Gene ID (NCBI) 3718
RRIDAB_2918888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52333
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

JAK3 is a non-receptor tyrosine kinase, predominantly expressed in hematopoietic cells and that has been implicated in the signal transduction of the common gamma chain subfamily of cytokine receptors. As a result, JAK3 plays an essential role in hematopoieisis during T cell development. JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. (PMID: 19747563, PMID: 33767339)

实验方案

Product Specific Protocols
WB protocol for JAK3 antibody 80331-1-RRDownload protocol
IHC protocol for JAK3 antibody 80331-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Immunol

Construction of disulfidptosis-based immune response prediction model with artificial intelligence and validation of the pivotal grouping oncogene c-MET in regulating T cell exhaustion

Authors - Pengping Li
View Article