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验证数据展示

  • WB analysis using 66922-2-Ig

    WB analysis using 66922-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 66922-2-Ig

    IHC staining of human skin cancer using 66922-2-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ITGB4 antibody included
  • IHC staining of human skin cancer using 66922-2-Ig

    IHC staining of human skin cancer using 66922-2-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ITGB4 antibody included
  • IF Staining of A431 using 66922-2-Ig

    IF Staining of A431 using 66922-2-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (66922-2-Ig, Clone: 1C2A11 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Integrin Beta 4 Monoclonal antibody

Integrin Beta 4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66922-2-Ig

产品说明书

CloneNo. 1C2A11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ITGB4, 1C2A11, CD104, GP150, Integrin beta-4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, BxPC-3 cells, SW 1990 cells, SKOV-3 cells, MCF-7 cells, Calu-3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ITGB4 Ready-To-Use IHC Kit. ITGB4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66922-2-Ig targets Integrin Beta 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17395

Product name: Recombinant human Integrin beta-4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 27-354 aa of BC126411

Sequence: ANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, beta 4
别名 ITGB4, 1C2A11, CD104, GP150, Integrin beta-4
计算分子量 1752 aa, 195 kDa
观测分子量 200-210 kDa
GenBank蛋白编号BC126411
基因名称 Integrin beta 4
Gene ID (NCBI) 3691
RRIDAB_2882249
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP16144
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

实验方案

Product Specific Protocols
IF protocol for Integrin Beta 4 antibody 66922-2-IgDownload protocol
IHC protocol for Integrin Beta 4 antibody 66922-2-IgDownload protocol
WB protocol for Integrin Beta 4 antibody 66922-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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