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1X10^6 human peripheral blood lymphocytes were surface stained with 1 ug Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black), or stained with 1 ug Anti-Human CD4 (65143-1-Ig, Clone: RPA-T4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green). Cells were not fixed.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.