• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite®594-conjugated ITM2C Monoclonal antibody

ITM2C Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, canine

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E8G11

Cat No : CL594-60095

Print datasheet

Synonyms

BRI3, BRICD2C, Cerebral protein 14, E25, E25C, integral membrane protein 2C, ITM2C, ITM3, PSEC0047, Transmembrane protein BRI3

验证数据展示

  • IF Staining of MDCK using CL594-60095

    IF Staining of MDCK using CL594-60095

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using the CoraLite®594-conjugated version of this antibody, CL594-60095 (ITM2C antibody), at dilution of 1:100.

经过测试的应用

Positive IF detected inMDCK cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60095-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60095 targets ITM2C in IF applications and shows reactivity with human, canine samples.

Tested Applications IF/ICC
Tested Reactivity human, canine
Immunogen ITM2C fusion protein Ag4409 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name integral membrane protein 2C
Synonyms BRI3, BRICD2C, Cerebral protein 14, E25, E25C, integral membrane protein 2C, ITM2C, ITM3, PSEC0047, Transmembrane protein BRI3
Calculated Molecular Weight230aa,26 kDa; 267aa,30 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC025742
Gene Symbol ITM2C
Gene ID (NCBI) 81618
RRIDAB_2883421
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQX7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Integral membrane protein 2C (ITM2C) is a type II integral transmembrane protein belonging to a family composed of at least two other members, ITM2A and ITM2B. ITM2C, often called transmembrane protein BRI3, is highly expressed in brain tissue. Yeast two-hybrid screen system revealed that BRI3 could interact with beta-secretase beta-amyloid protein converting enzyme (BACE)1 and the microtubule-destabilizing protein SCG10 (STMN2). It may play a role in TNF-induced cell death and neuronal differentiation. BRI3 was found to inhibit the various processing of amyloid precursor protein (APP) by blocking the access of alpha- and beta-secretases to APP, competitive inhibition of APP processing by BRI3 may provide a new approach to Alzheimer disease (AD) therapy and prevention.

实验方案

Product Specific Protocols
IF protocol for CL594 ITM2C antibody CL594-60095Download protocol
Standard Protocols
Click here to view our Standard Protocols