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验证数据展示

  • WB analysis using 21141-1-AP

    WB analysis using 21141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21141-1-AP (ITIH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ITIH1 Polyclonal antibody

ITIH1 Polyclonal Antibody for WB, ELISA
Cat No. 21141-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Inter-alpha-trypsin inhibitor heavy chain H1, Serum-derived hyaluronan-associated protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse auricular cartilage tissue, mouse cartilage tissue, rat auricular cartilage tissue
Planning an IHC experiment? We recommend our IHCeasy® ITIH1 Ready-To-Use IHC Kit. ITIH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21141-1-AP targets ITIH1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15598

Product name: Recombinant human ITIH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 608-811 aa of BC069464

Sequence: SMSIRGMADQDGLKPTIDKPSEDSPPLEMLGPRRTFVLSALQPSPTHSSSNTQRLPDRVTGVDTDPHFIIHVPQKEDTLCFNINEEPGVILSLVQDPNTGFSVNGQLIGNKARSPGQHDGTYFGRLGIANPATDFQLEVTPQNITLNPGFGGPVFSWRDQAVLRQDGVVVTINKKRNLVVSVDDGGTFEVVLHRVWKGSSVHQD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inter-alpha (globulin) inhibitor H1
别名 Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Inter-alpha-trypsin inhibitor heavy chain H1, Serum-derived hyaluronan-associated protein
计算分子量 911 aa, 101 kDa
观测分子量70-76 kDa
GenBank蛋白编号BC069464
基因名称 ITIH1
Gene ID (NCBI) 3697
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19827
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITIH1 (inter-alpha-trypsin inhibitor heavy chain 1), also named as IGHEP1 and SHAP. It is a key component of the inter-alpha-trypsin inhibitor (ITI) family, which plays a crucial role in extracellular matrix (ECM) stabilization and inflammation regulation. ITIH1 forms a complex with bikunin (a light chain) and other heavy chains (ITIH2, ITIH3, ITIH4), contributing to the stabilization of hyaluronan-rich matrices. The calculated molecular weight of the ITIH1 mature form is 101 kDa, but the actual observed molecular weight is about 76 kDa (PMID: 9677337).

实验方案

Product Specific Protocols
WB protocol for ITIH1 antibody 21141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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