验证数据展示
经过测试的应用
Positive WB detected in | mouse auricular cartilage tissue, mouse cartilage tissue, rat auricular cartilage tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21141-1-AP targets ITIH1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | ITIH1 fusion protein Ag15598 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | inter-alpha (globulin) inhibitor H1 |
别名 | Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Inter-alpha-trypsin inhibitor heavy chain H1, Serum-derived hyaluronan-associated protein |
计算分子量 | 911 aa, 101 kDa |
观测分子量 | 70-76 kDa |
GenBank蛋白编号 | BC069464 |
基因名称 | ITIH1 |
Gene ID (NCBI) | 3697 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P19827 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITIH1 (inter-alpha-trypsin inhibitor heavy chain 1), also named as IGHEP1 and SHAP. It is a key component of the inter-alpha-trypsin inhibitor (ITI) family, which plays a crucial role in extracellular matrix (ECM) stabilization and inflammation regulation. ITIH1 forms a complex with bikunin (a light chain) and other heavy chains (ITIH2, ITIH3, ITIH4), contributing to the stabilization of hyaluronan-rich matrices. The calculated molecular weight of the ITIH1 mature form is 101 kDa, but the actual observed molecular weight is about 76 kDa (PMID: 9677337).
实验方案
Product Specific Protocols | |
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WB protocol for ITIH1 antibody 21141-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |