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验证数据展示

  • WB analysis using 85072-5-RR

    WB analysis using 85072-5-RR

    IFN alpha treated and untreated Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85072-5-RR (IRF7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 85072-5-RR

    FC experiment of Jurkat using 85072-5-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug IRF7 Recombinant antibody (85072-5-RR, Clone:242613C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85072-5-RR

    Affinity and Kinetic Characterization of 85072-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85072-5-RR against Human IRF7 were performed. The affinity constant is 13.1 pM.

IRF7 Recombinant antibody

IRF7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85072-5-RR

产品说明书

CloneNo. 242613C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

242613C2, IRF 7, IRF 7H, IRF-7, IRF7A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inIFN alpha treated Jurkat cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy IRF7 Ready-To-Use IHC Kit. IRF7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85072-5-RR targets IRF7 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 IRF7 fusion protein Ag18059 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IRF 7
别名 242613C2, IRF 7, IRF 7H, IRF-7, IRF7A
计算分子量 516 aa, 56 kDa
观测分子量 65 kDa
GenBank蛋白编号BC136555
基因名称 IRF7
Gene ID (NCBI) 3665
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92985
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF-7 (IFN regulatory factor 7) is a member of the IFN regulatory transcription factor (IRF) family. IRF-7 has been shown to play a role in the transcriptional activation of virus-inducible cellular proteins, including IFN beta chain proteins. Inducible expression of IRF-7 is largely restricted to lymphoid tissue. Four transcript variants encoding distinct isoforms (A,B,C and D) have been identified for this (PMID:9786932).The active IRF7A exists as a dimer form ~80 kDa(PMID:11073981). The MW 67-70 kDa has been reported in some papers (PMID:9786932; 22951831).Various posttranslational modifications of IRF7 including phosphorylation, ubiquitination, sumoylation and acetylation are identified (PMID:22951831).

实验方案

Product Specific Protocols
WB protocol for IRF7 antibody 85072-5-RRDownload protocol
FC protocol for IRF7 antibody 85072-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols