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Host
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 85072-5-RR (same clone as 85072-5-PBS)

    WB analysis using 85072-5-RR (same clone as 85072-5-PBS)

    IFN alpha treated and untreated Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85072-5-RR (IRF7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85072-5-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 85072-5-RR (same clone as 85072-5-PBS)

    FC experiment of Jurkat using 85072-5-RR (same clone as 85072-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug IRF7 Recombinant antibody (85072-5-RR, Clone:242613C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85072-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85072-5-RR

    Affinity and Kinetic Characterization of 85072-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85072-5-RR against Human IRF7 were performed. The affinity constant is 13.1 pM.

IRF7 Recombinant antibody, PBS Only

IRF7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 85072-5-PBS

产品说明书

CloneNo. 242613C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

242613C2, IRF 7, IRF 7H, IRF-7, IRF7A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IRF7 Ready-To-Use IHC Kit. IRF7 primary antibody included.

产品信息

85072-5-PBS targets IRF7 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 IRF7 fusion protein Ag18059 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IRF 7
别名 242613C2, IRF 7, IRF 7H, IRF-7, IRF7A
计算分子量 516 aa, 56 kDa
观测分子量 65 kDa
GenBank蛋白编号BC136555
基因名称 IRF7
Gene ID (NCBI) 3665
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92985
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IRF-7 (IFN regulatory factor 7) is a member of the IFN regulatory transcription factor (IRF) family. IRF-7 has been shown to play a role in the transcriptional activation of virus-inducible cellular proteins, including IFN beta chain proteins. Inducible expression of IRF-7 is largely restricted to lymphoid tissue. Four transcript variants encoding distinct isoforms (A,B,C and D) have been identified for this (PMID:9786932).The active IRF7A exists as a dimer form ~80 kDa(PMID:11073981). The MW 67-70 kDa has been reported in some papers (PMID:9786932; 22951831).Various posttranslational modifications of IRF7 including phosphorylation, ubiquitination, sumoylation and acetylation are identified (PMID:22951831).