验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, PC-12 cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
86068-3-RR targets IRAK1 in WB, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, rat |
免疫原 | IRAK1 fusion protein Ag0728 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | interleukin-1 receptor-associated kinase 1 |
别名 | EC:2.7.11.1, Interleukin-1 receptor-associated kinase 1, IRAK, IRAK 1, IRAK-1 |
计算分子量 | 77 kDa |
观测分子量 | 68-80 kDa |
GenBank蛋白编号 | BC014963 |
基因名称 | IRAK1 |
Gene ID (NCBI) | 3654 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P51617 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)
实验方案
Product Specific Protocols | |
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WB protocol for IRAK1 antibody 86068-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |