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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86068-3-RR (same clone as 86068-3-PBS)

    WB analysis using 86068-3-RR (same clone as 86068-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86068-3-RR (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86068-3-PBS in a different storage buffer formulation.

  • WB analysis of PC-12 using 86068-3-RR (same clone as 86068-3-PBS)

    WB analysis of PC-12 using 86068-3-RR (same clone as 86068-3-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 86068-3-RR (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86068-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86068-3-RR (same clone as 86068-3-PBS)

    IF Staining of HeLa using 86068-3-RR (same clone as 86068-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IRAK1 antibody (86068-3-RR, Clone: 250691C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86068-3-PBS in a different storage buffer formulation.

  • IF Staining of SKOV-3 using 86068-3-RR (same clone as 86068-3-PBS)

    IF Staining of SKOV-3 using 86068-3-RR (same clone as 86068-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using IRAK1 antibody (86068-3-RR, Clone: 250691C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86068-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86068-3-RR

    Affinity and Kinetic Characterization of 86068-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86068-3-RR against Human IRAK1 were performed. The affinity constant is 2.50 nM.

IRAK1 Recombinant antibody, PBS Only

IRAK1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86068-3-PBS

产品说明书

CloneNo. 250691C5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Indirect ELISA

别名

EC:2.7.11.1, Interleukin-1 receptor-associated kinase 1, IRAK, IRAK 1, IRAK-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IRAK1 Ready-To-Use IHC Kit. IRAK1 primary antibody included.

产品信息

86068-3-PBS targets IRAK1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 IRAK1 fusion protein Ag0728 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin-1 receptor-associated kinase 1
别名 EC:2.7.11.1, Interleukin-1 receptor-associated kinase 1, IRAK, IRAK 1, IRAK-1
计算分子量 77 kDa
观测分子量68-80 kDa
GenBank蛋白编号BC014963
基因名称 IRAK1
Gene ID (NCBI) 3654
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP51617
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)