验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | A431 cells |
| Positive FC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67962 targets IPO5 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 |
CatNo: Ag30955 Product name: Recombinant human IPO5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 22-150 aa of BC001497 Sequence: DNVVRKQAEETYENIPGQSKITFLLQAIRNTTAAEEARQMAAVLLRRLLSSAFDEVYPALPSDVQTAIKSELLMIIQMETQSSMRKKVCDIAAELARNLIDEDGNNQWPEGLKFLFDSVSSQNVGLREA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | importin 5 |
| 别名 | IPO5, Karyopherin beta 3, Karyopherin beta-3, KPNB3, Ran binding protein 5, Ran-binding protein 5, RANBP5, 1E2B9, IMB3, Imp5, importin 5, Importin subunit beta 3, Importin subunit beta-3, Importin-5 |
| 观测分子量 | 124 kDa |
| GenBank蛋白编号 | BC001497 |
| 基因名称 | IPO5 |
| Gene ID (NCBI) | 3843 |
| RRID | AB_2934600 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00410 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IPO5 is a member of the importin beta family. Structurally, the protein adopts the shape of a right-hand solenoid and is composed of 24 HEAT repeats. IPO5 facilitates cytoplasmic polyadenylation element-binding protein CPEB3 translocation by binding to the RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3-IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IPO5 antibody CL488-67962 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |