验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 67549-1-Ig
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-67549 targets IPO4 in IF/ICC applications and shows reactivity with Human samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | Human |
免疫原 | IPO4 fusion protein Ag2281 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | importin 4 |
别名 | FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4 |
计算分子量 | 1081 aa, 118 kDa |
观测分子量 | 120 kDa |
GenBank蛋白编号 | BC003690 |
基因名称 | IPO4 |
Gene ID (NCBI) | 79711 |
RRID | AB_2920156 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q8TEX9 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 IPO4 antibody CL594-67549 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |