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验证数据展示

  • WB analysis using 84467-2-RR (same clone as 84467-2-PBS)

    WB analysis using 84467-2-RR (same clone as 84467-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84467-2-RR (INSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84467-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01349-2

    Sandwich ELISA standard curve of MP01349-2

    Sandwich ELISA standard curve of MP01349-2, Human INSR Recombinant Matched Antibody Pair - PBS only. 84467-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00007. 84467-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • Sample test of MP01349-2

    Sample test of MP01349-2

    The mean INSR concentration was determined to be 72.33 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load and 87.72 ng/mL in HT-1080 cell extract based on a 2.50 mg/mL extract load.

  • IF Staining of MCF-7 using 84467-2-RR (same clone as 84467-2-PBS)

    IF Staining of MCF-7 using 84467-2-RR (same clone as 84467-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using INSR/CD220 antibody (84467-2-RR, Clone: 241687H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84467-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01349-1

    Cytometric bead array standard curve of MP01349-1

    Cytometric bead array standard curve of MP01349-1, INSR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84467-2-PBS. Detection antibody: 84467-1-PBS. Standard: RP00007. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 84467-2-RR

    Affinity and Kinetic Characterization of 84467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84467-2-RR against Human INSR were performed. The affinity constant is 0.189 nM.

INSR/CD220 Recombinant antibody, PBS Only (Capture/Detector)

INSR/CD220 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84467-2-PBS

产品说明书

CloneNo. 241687H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

INSR, 241687H7, CD220, EC:2.7.10.1, HHF5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84467-2-PBS targets INSR/CD220 as part of a matched antibody pair:

MP01349-1: 84467-2-PBS capture and 84467-1-PBS detection (validated in Cytometric bead array)

MP01349-2: 84467-1-PBS capture and 84467-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 INSR
别名 INSR, 241687H7, CD220, EC:2.7.10.1, HHF5
观测分子量95 kDa
GenBank蛋白编号BC117172
基因名称 INSR
Gene ID (NCBI) 3643
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06213
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).