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验证数据展示

  • WB analysis using 84467-2-RR

    WB analysis using 84467-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84467-2-RR (INSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 84467-2-RR

    IHC staining of human breast cancer using 84467-2-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84467-2-RR (INSR/CD220 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 84467-2-RR

    IF Staining of MCF-7 using 84467-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using INSR/CD220 antibody (84467-2-RR, Clone: 241687H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84467-2-RR

    Affinity and Kinetic Characterization of 84467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84467-2-RR against Human INSR were performed. The affinity constant is 0.189 nM.

INSR/CD220 Recombinant monoclonal antibody

INSR/CD220 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84467-2-RR

产品说明书

CloneNo. 241687H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

INSR, 241687H7, CD220, EC:2.7.10.1, HHF5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293T cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84467-2-RR targets INSR/CD220 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 INSR
别名 INSR, 241687H7, CD220, EC:2.7.10.1, HHF5
观测分子量95 kDa
GenBank蛋白编号BC117172
基因名称 INSR
Gene ID (NCBI) 3643
RRIDAB_3671990
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06213
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).

实验方案

Product Specific Protocols
IF protocol for INSR/CD220 antibody 84467-2-RRDownload protocol
IHC protocol for INSR/CD220 antibody 84467-2-RRDownload protocol
WB protocol for INSR/CD220 antibody 84467-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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