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验证数据展示

  • FC experiment of HUVEC using CL488-98206-10

    FC experiment of HUVEC using CL488-98206-10

    1x10^6 HUVEC cells were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human IL-7 Rabbit RecAb (CL488-98206-10, Clone:252658G1) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • FC experiment of Jurkat using CL488-98206-10

    FC experiment of Jurkat using CL488-98206-10

    1x10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human IL-7 Rabbit RecAb (CL488-98206-10, Clone:252658G1) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

CoraLite® Plus 488 Anti-Human IL-7 Rabbit Recombinant Antibody

IL-7 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. CL488-98206-10

产品说明书

CloneNo. 252658G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

IL7, IL 7, interleukin 7, Interleukin-7

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHUVEC cells, Jurkat cells
For other applications, we recommend the unconjugated version of this antibody, 98206-10-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul / test per 10^6 cells in a 100 µl suspension or 5 ul / test per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-98206-10 targets IL-7 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo:

Product name: HumanKine® recombinant human IL-7 protein

Source: -derived,

Tag:

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 7
别名 IL7, IL 7, interleukin 7, Interleukin-7
基因名称 IL-7
Gene ID (NCBI) 3574
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13232
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-7 antibody CL488-98206-10Download protocol
Standard Protocols
Click here to view our Standard Protocols
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