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验证数据展示

  • FC experiment of Raji using 98206-10-RR

    FC experiment of Raji using 98206-10-RR

    1x10^6 Raji cells were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit RecAb (98206-10-RR, Clone: 252658G1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IHC staining of human tonsil using 98206-10-RR

    IHC staining of human tonsil using 98206-10-RR

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 98206-10-RR (IL-7 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsil using 98206-10-RR

    IHC staining of human tonsil using 98206-10-RR

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 98206-10-RR (IL-7 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Anti-Human IL-7 Rabbit Recombinant Antibody

IL-7 Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98206-10-RR

产品说明书

CloneNo. 252658G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra)

别名

IL7, IL 7, interleukin 7, Interleukin-7

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inRaji cells, U87-MG

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98206-10-RR targets IL-7 in IHC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo:

Product name: HumanKine® recombinant human IL-7 protein

Source: -derived,

Tag:

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 7
别名 IL7, IL 7, interleukin 7, Interleukin-7
基因名称 IL-7
Gene ID (NCBI) 3574
RRIDAB_3745873
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13232
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)

实验方案

Product Specific Protocols
FC protocol for IL-7 antibody 98206-10-RRDownload protocol
IHC protocol for IL-7 antibody 98206-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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