Cytometric bead array of HT-1080 cells supernatant using MP50032-1, IL-6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. HT-1080 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 6 days in normal culture media or IL-17A (Cat.NO. HZ-1113, 40 ng/mL) containing media. 20 μL of the supernatant from day 1, 2, 3 and day 6 was analyzed using MP50032-1 (IL-6 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time.Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

Cytometric bead array standard curve of MP50032-1, IL-6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. Standard:HZ-1019. Range: 0.098-12.5 ng/mL.

Serum of sixteen individual healthy human donors was measured. The IL-6 concentration of detected samples was determined to be 73.8 pg/mL with a range of ND-113.9 pg/mL.

Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 day or 3 days. For the PBMC supernatant cultured for 1 day, the mean IL-6 concentration was determined to be 0.1 ng/mL in unstimulated PBMC supernatant, 19.0 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 3 days, the mean IL-6 concentration was determined to be 0.1 ng/mL in unstimulated PBMC supernatant, 39.0 ng/mL in PHA stimulated PBMC supernatant.

Sandwich ELISA standard curve of MP50032-1, IL6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. Standard: HZ-1019. Range: 7.8-500 pg/mL.