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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98336-2-RR

    FC experiment of human peripheral blood leukocytes using 98336-2-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-3RB/CD131 Rabbit RecAb (98336-2-RR, Clone: 241823B12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98336-2-RR

    FC experiment of human peripheral blood leukocytes using 98336-2-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-3RB/CD131 Rabbit RecAb (98336-2-RR, Clone: 241823B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibody

IL-3RB/CD131 Uni-rAb® Recombinant Antibody for FC
Cat No. 98336-2-RR

产品说明书

CloneNo. 241823B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CSF2RB, CD131, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit, IL3RB

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
PE
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes, Transfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98336-2-RR targets IL-3RB/CD131 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1662

Product name: Recombinant Human IL-3RB/CD131 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 17-443 aa of NM_000395.3

Sequence: WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
别名 CSF2RB, CD131, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit, IL3RB
计算分子量97kDa
GenBank蛋白编号NM_000395.3
基因名称 CSF2RB
Gene ID (NCBI) 1439
RRIDAB_3745986
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP32927-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

IL-3RB/CD131, also known as CSF2RB, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. CD131 is expressed on various immune cells, including hematopoietic progenitor cells, granulocytes, monocytes, macrophages, eosinophils, and mast cells, which plays a crucial role in mediating the effects of cytokines (PMID: 18412971). Tregs of healthy donors constitutively transcribe CSF2RB, but protein expression was much higher after TCR stimulation (PMID: 36532080, 3557440).

实验方案

Product Specific Protocols
FC protocol for IL-3RB/CD131 antibody 98336-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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