验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PMA and ionomycin treated rat splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 98046-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-98046 targets IL-2 in FC (Intra) applications and shows reactivity with rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | rat |
| 免疫原 | Fusion Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 2 |
| 别名 | Il2, interleukin 2, 240264C7 |
| 计算分子量 | 18 kDa |
| GenBank蛋白编号 | NM_053836 |
| 基因名称 | Il2 |
| Gene ID (NCBI) | 116562 |
| RRID | AB_3672421 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17108 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 IL-2 antibody CL405-98046 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |