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验证数据展示

  • FC experiment of human PBMCs using FITC-65286

    FC experiment of human PBMCs using FITC-65286

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-10 (FITC-65286, Clone:7B8G3) or Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using FITC-65286

    FC experiment of human PBMCs using FITC-65286

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-10 (FITC-65286, Clone:7B8G3) (red) or Mouse IgG2b Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-10 (FITC-65286, Clone:7B8G3) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

FITC Plus Anti-Human IL-10 (7B8G3)

IL-10 Monoclonal Antibody for FC (Intra)
Cat No. FITC-65286

产品说明书

CloneNo. 7B8G3

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

FC (Intra)

别名

IL-10, IL-10,IL10, IL10A, interleukin 10, Interleukin-10, TGIF, 7B8G3, CSIF, IL 10, IL10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC Plus
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65286 targets IL-10 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 IL-10 fusion protein HZ-1145 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 10
别名 IL-10, IL-10,IL10, IL10A, interleukin 10, Interleukin-10, TGIF, 7B8G3, CSIF, IL 10, IL10
计算分子量 21 kDa
GenBank蛋白编号BC104252
基因名称 IL-10
Gene ID (NCBI) 3586
RRIDAB_3085009
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

实验方案

Product Specific Protocols
FC protocol for FITC Plus IL-10 antibody FITC-65286Download protocol
Standard Protocols
Click here to view our Standard Protocols