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CoraLite® Plus 647 Anti-Human IL-10 (7B8G3)

IL-10 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

7B8G3

Cat No : CL647-65286

Print datasheet

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

验证数据展示

  • FC experiment of human PBMCs using CL647-65286

    FC experiment of human PBMCs using CL647-65286

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-10 (CL647-65286, Clone:7B8G3) or Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL647-65286

    FC experiment of human PBMCs using CL647-65286

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-10 (CL647-65286, Clone:7B8G3) (red) or Mouse IgG2b Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human IL-10 (CL647-65286, Clone:7B8G3) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

经过测试的应用

Positive FC detected inLPS and Brefeldin A treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65286 targets IL-10 in FC applications and shows reactivity with Human samples.

Tested Applications FC
Tested Reactivity Human
Immunogen IL-10 fusion protein HZ-1145 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin 10
Synonyms CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC104252
Gene Symbol IL10
Gene ID (NCBI) 3586
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IL-10 antibody CL647-65286Download protocol
Standard Protocols
Click here to view our Standard Protocols