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验证数据展示

  • IHC staining of human tonsillitis using 11541-1-AP

    IHC staining of human tonsillitis using 11541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11541-1-AP (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 11541-1-AP

    IF Staining of human tonsillitis using 11541-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IgG Lambda Light Chain antibody (11541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human plasma using 11541-1-AP

    WB analysis of human plasma using 11541-1-AP

    human plasma (0.3 ug) were subjected to SDS PAGE followed by western blot with 11541-1-AP (IgG light chain Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 11541-1-AP

    IHC staining of human tonsillitis using 11541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11541-1-AP (Ig Lambda Light Chain antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 11541-1-AP

    IHC staining of human tonsillitis using 11541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11541-1-AP (Ig Lambda Light Chain antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Ramos using 11541-1-AP

    FC experiment of Ramos using 11541-1-AP

    1x10^6 Ramos cells were intracellularly stained with 0.4 ug IgG Lambda Light Chain Polyclonal antibody (11541-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Ig Lambda Light Chain Polyclonal antibody

Ig Lambda Light Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 11541-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA and More (2)

别名

IgG Lambda Light Chain, IGL, IgG, IGL-, immunoglobulin lambda locus

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma tissue, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive FC (Intra) detected inRamos cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11541-1-AP targets Ig Lambda Light Chain in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, IP, ELISA
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag2064

Product name: Recombinant human IGL- protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-237 aa of BC020233

Sequence: MAWSPLLLTLLAHCTGSWAQSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYGNSNRPSGVPDRFSGSKSGTSASLAITGLQAEDEADYYCQSYDSSLSGFVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin lambda locus
别名 IgG Lambda Light Chain, IGL, IgG, IGL-, immunoglobulin lambda locus
计算分子量 237 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC020233
基因名称 IgG Lambda Light Chain
Gene ID (NCBI) 3535
RRIDAB_2877775
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the lambda light chain of human immunoglobulin.

实验方案

Product Specific Protocols
FC protocol for Ig Lambda Light Chain antibody 11541-1-APDownload protocol
IF protocol for Ig Lambda Light Chain antibody 11541-1-APDownload protocol
IHC protocol for Ig Lambda Light Chain antibody 11541-1-APDownload protocol
WB protocol for Ig Lambda Light Chain antibody 11541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Cell Death Dis

SLC44A2 negatively regulates mitochondrial fatty acid oxidation to suppress colorectal progression by blocking the MUL1-CPT2 interaction.

Authors - Ying Yang
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Proc Natl Acad Sci U S A

Biopsy-resolved cryo-EM structures of amyloid fibrils provide molecular insights into AL amyloidosis.

Authors - Yuxuan Yao
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humanWB

Cancer Immunol Immunother

A high proportion of CD38 (high) CD16 (low) NK cells in colorectal cancer can interrupt immune surveillance and favor tumor growth

Authors - Xueling Wang
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humanWB,IHC,IF,ELISA

World J Diabetes

Nephroprotective mechanism of Kunkui Baoshen decoction in diabetic kidney disease: Targeting the HERC2/NCOA4-mediated autophagy-dependent ferroptosis pathway.

Authors - Si-Yuan Song
View Article
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