验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84092-3 targets IGFBP2 in IF/ICC applications and shows reactivity with mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg1196 Product name: Recombinant Mouse IGFBP2 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 35-305 aa of NM_008342.3 Sequence: EVLFRCPPCTPERLAACGPPPDAPCAELVREPGCGCCSVCARQEGEACGVYIPRCAQTLRCYPNPGSELPLKALVTGAGTCEKRRVGTTPQQVADSDDDHSEGGLVENHVDGTMNMLGGGSSAGRKPLKSGMKELAVFREKVNEQHRQMGKGAKHLSLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDDRGPLEHLYSLHIPNCDKHGRYNLKQCKMSLNGQRGECWCVNPNTGKPIQGAPTIRGDPECHLFYNEQQETGGAHAQSVQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | insulin-like growth factor binding protein 2 |
| 别名 | IBP-2, IGF-binding protein 2, IGFBP-2, Insulin-like growth factor-binding protein 2, mIGFBP-2 |
| 计算分子量 | 33kDa |
| GenBank蛋白编号 | NM_008342.3 |
| 基因名称 | Igfbp2 |
| Gene ID (NCBI) | 16008 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P47877 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGFBP2 is a key member of the IGFBP family that is highly expressed in embryonic and fetal tissues and decreases significantly after birth. IGFBP2 mediates IGF-independent tumorigenesis by participating in intracellular and nuclear regulatory networks and may serve as an excellent prognostic biomarker in various cancers (PMID: 35546443).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 IGFBP2 antibody CL488-84092-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
