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Host
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Species Reactivity
0
Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 66283-1-Ig (same clone as 66283-1-PBS)

    WB analysis using 66283-1-Ig (same clone as 66283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGF1R antibody included
  • IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGF1R antibody included

IGF1R/CD221 Monoclonal antibody, PBS Only

IGF1R/CD221 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66283-1-PBS

产品说明书

CloneNo. 3C6A11

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IGF1R Ready-To-Use IHC Kit. IGF1R primary antibody included.

产品信息

66283-1-PBS targets IGF1R/CD221 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IGF I receptor
别名 IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR
计算分子量 155 kDa
观测分子量 130-140 kDa
GenBank蛋白编号NM_000875
基因名称 IGF1R
Gene ID (NCBI) 3480
RRIDAB_2881666
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08069
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

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