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验证数据展示

  • WB analysis of rat plasma using 84782-5-RR (same clone as 84782-5-PBS)

    WB analysis of rat plasma using 84782-5-RR (same clone as 84782-5-PBS)

    rat plasma were subjected to SDS PAGE followed by western blot with 84782-5-RR (IGF-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84782-5-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 84782-5-RR (same clone as 84782-5-PBS)

    FC experiment of Transfected HEK-293T using 84782-5-RR (same clone as 84782-5-PBS)

    1x10^6 HEK-293T cells or IGF1 Transfected HEK-293T cells were intracellularly stained with 0.25 ug IGF1 Recombinant antibody (84782-5-RR, Clone:241890G6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84782-5-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 84782-5-RR (same clone as 84782-5-PBS)

    IHC staining of rat liver using 84782-5-RR (same clone as 84782-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84782-5-RR (IGF1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84782-5-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 84782-5-RR (same clone as 84782-5-PBS)

    IHC staining of rat liver using 84782-5-RR (same clone as 84782-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84782-5-RR (IGF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84782-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84782-5-RR

    Affinity and Kinetic Characterization of 84782-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84782-5-RR against Rat IGF1 were performed. The affinity constant is below 1 pM.

Rat IGF1 Recombinant monoclonal antibody, PBS Only

IGF1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84782-5-PBS

产品说明书

CloneNo. 241890G6

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84782-5-PBS targets IGF1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 rat
免疫原

CatNo: Eg1976

Product name: Recombinant Rat IGF1 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 23-92 aa of AAA41386

Sequence: GPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor 1
别名 241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin
计算分子量14kd
观测分子量12 kDa
GenBank蛋白编号AAA41386
基因名称 Igf1
Gene ID (NCBI) 24482
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08025
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

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