IGF1 Recombinant antibody

IGF1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84782-5-RR

产品说明书

CloneNo. 241890G6

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, FC (Intra), ELISA

241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrat plasma
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84782-5-RR targets IGF1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 rat
免疫原 IGF1 fusion protein Eg1976 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor 1
别名 241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin
计算分子量14kd
观测分子量12 kDa
GenBank蛋白编号AAA41386
基因名称 Igf1
Gene ID (NCBI) 24482
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08025
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

实验方案

Product Specific Protocols
WB protocol for IGF1 antibody 84782-5-RRDownload protocol
IHC protocol for IGF1 antibody 84782-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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