验证数据展示
经过测试的应用
Positive WB detected in | rat plasma |
Positive IHC detected in | rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | Transfected HEK-293T cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84782-5-RR targets IGF1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with rat samples.
经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
经测试反应性 | rat |
免疫原 | IGF1 fusion protein Eg1976 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | insulin-like growth factor 1 |
别名 | 241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin |
计算分子量 | 14kd |
观测分子量 | 12 kDa |
GenBank蛋白编号 | AAA41386 |
基因名称 | Igf1 |
Gene ID (NCBI) | 24482 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P08025 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.
实验方案
Product Specific Protocols | |
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WB protocol for IGF1 antibody 84782-5-RR | Download protocol |
IHC protocol for IGF1 antibody 84782-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |