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验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-17460

    IF Staining of hTERT-RPE1 using CL488-17460

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT140 antibody (CL488-17460) at dilution of 1:200, ARL13B antibody (17711-1-AP, red).

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CoraLite® Plus 488-conjugated IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for IF/ICC
Cat No. CL488-17460

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

c305C8.4, c380F5.1, DKFZp564L232, FLJ10306, FLJ30571, gs114, IFT140, KIAA0590, WDTC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 17460-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17460 targets IFT140 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 IFT140 fusion protein Ag11119 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 140 homolog (Chlamydomonas)
别名 c305C8.4, c380F5.1, DKFZp564L232, FLJ10306, FLJ30571, gs114, IFT140, KIAA0590, WDTC2
计算分子量 1462 aa, 165 kDa
GenBank蛋白编号BC035577
基因名称 IFT140
Gene ID (NCBI) 9742
RRIDAB_2919147
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RY7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFT140 antibody CL488-17460Download protocol
Standard Protocols
Click here to view our Standard Protocols