- Featured Product
- KD/KO Validated
IFT140 Polyclonal antibody
IFT140 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, rat testis tissue, HepG2 cells, mouse testis tissue |
Positive IP detected in | rat testis tissue |
Positive IHC detected in | mouse testis tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C2C12 cells, hTERT-RPE1 cells, mouse testis tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17460-1-AP targets IFT140 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, zebrafish, canine |
Immunogen | IFT140 fusion protein Ag11119 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | intraflagellar transport 140 homolog (Chlamydomonas) |
Synonyms | c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2 |
Calculated Molecular Weight | 1462 aa, 165 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | BC035577 |
Gene Symbol | IFT140 |
Gene ID (NCBI) | 9742 |
RRID | AB_2295648 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96RY7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for IFT140 antibody 17460-1-AP | Download protocol |
IHC protocol for IFT140 antibody 17460-1-AP | Download protocol |
IF protocol for IFT140 antibody 17460-1-AP | Download protocol |
IP protocol for IFT140 antibody 17460-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Genet Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. | ||
Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. | ||
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Nat Commun A distal centriolar protein network controls organelle maturation and asymmetry. | ||
Nat Commun Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling. | ||
Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. |