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验证数据展示

  • WB analysis of HeLa using 82947-7-RR (same clone as 82947-7-PBS)

    WB analysis of HeLa using 82947-7-RR (same clone as 82947-7-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82947-7-RR (same clone as 82947-7-PBS)

    WB analysis of HepG2 using 82947-7-RR (same clone as 82947-7-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 82947-7-RR (same clone as 82947-7-PBS)

    IF Staining of hTERT-RPE1 using 82947-7-RR (same clone as 82947-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82947-7-PBS

    Affinity and Kinetic Characterization of 82947-7-PBS

    Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

IFT140 Recombinant monoclonal antibody, PBS Only

IFT140 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82947-7-PBS

产品说明书

CloneNo. 230231D5

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82947-7-PBS targets IFT140 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag11119

Product name: Recombinant human IFT140 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1114-1462 aa of BC035577

Sequence: AEDLDETSDPALLARCSDFFIEHSQYERAVELLLAARKYQEALQLCLGQNMSITEEMAEKMTVAKDSSDLPEESRRELLEQIADCCMRQGSYHLATKKYTQAGNKLKAMRALLKSGDTEKITFFASVSRQKEIYIMAANYLQSLDWRKEPEIMKNIIGFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLAKAKAKSPLDQETRLAQLQSRMALVKRFIQARRTYTEDPKESIKQCELLLEEPDLDSTIRIGDVYGFLVEHYVRKEEYQTAYRFLEEMRRRLPLANMSYYVSPQAVDAVHRGLGLPLPRTVPEQVRHNSMEDARELDEEVVEEADDDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 intraflagellar transport 140 homolog (Chlamydomonas)
别名 WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5
计算分子量 1462 aa, 165 kDa
GenBank蛋白编号BC035577
基因名称 IFT140
Gene ID (NCBI) 9742
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96RY7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).

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