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验证数据展示

  • WB analysis of HeLa using 82947-7-RR

    WB analysis of HeLa using 82947-7-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 82947-7-RR

    WB analysis of HepG2 using 82947-7-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 82947-7-RR

    IF Staining of hTERT-RPE1 using 82947-7-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Affinity and Kinetic Characterization of 82947-7-RR

    Affinity and Kinetic Characterization of 82947-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

IFT140 Recombinant monoclonal antibody

IFT140 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82947-7-RR

产品说明书

CloneNo. 230231D5

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inStarvation treated hTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82947-7-RR targets IFT140 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag11119

Product name: Recombinant human IFT140 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1114-1462 aa of BC035577

Sequence: AEDLDETSDPALLARCSDFFIEHSQYERAVELLLAARKYQEALQLCLGQNMSITEEMAEKMTVAKDSSDLPEESRRELLEQIADCCMRQGSYHLATKKYTQAGNKLKAMRALLKSGDTEKITFFASVSRQKEIYIMAANYLQSLDWRKEPEIMKNIIGFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLAKAKAKSPLDQETRLAQLQSRMALVKRFIQARRTYTEDPKESIKQCELLLEEPDLDSTIRIGDVYGFLVEHYVRKEEYQTAYRFLEEMRRRLPLANMSYYVSPQAVDAVHRGLGLPLPRTVPEQVRHNSMEDARELDEEVVEEADDDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 intraflagellar transport 140 homolog (Chlamydomonas)
别名 WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5
计算分子量 1462 aa, 165 kDa
观测分子量150-165 kDa
GenBank蛋白编号BC035577
基因名称 IFT140
Gene ID (NCBI) 9742
RRIDAB_3670689
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96RY7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).

实验方案

Product Specific Protocols
IF protocol for IFT140 antibody 82947-7-RRDownload protocol
WB protocol for IFT140 antibody 82947-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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