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验证数据展示

  • IF Staining of K-562 using CL594-60074

    IF Staining of K-562 using CL594-60074

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite®594 IFITM1-Specific antibody (CL594-60074, Clone: 5B5E2 ) at dilution of 1:200.

  • IF Staining of K-562 using CL594-60074

    IF Staining of K-562 using CL594-60074

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite®594 IFITM1-Specific antibody (CL594-60074, Clone: 5B5E2 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated IFITM1-Specific Monoclonal antibody

IFITM1-Specific Monoclonal Antibody for IF/ICC
Cat No. CL594-60074

产品说明书

CloneNo. 5B5E2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC

别名

IFITM1, DSPA2a, Dispanin subfamily A member 2a, CD225, 927

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy IFITM1 Ready-To-Use IHC Kit. IFITM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60074-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60074 targets IFITM1-Specific in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag2320

Product name: Recombinant human IFITM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-125 aa of BC000897

Sequence: MHKEEHEVAVLGAPPSTILPRSTVINIHSETSVPDHVVWSLFNTLFLNWCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGILMTIGFILLLVFGSVTVYHIMLQIIQEKRGY

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 interferon induced transmembrane protein 1 (9-27)
别名 IFITM1, DSPA2a, Dispanin subfamily A member 2a, CD225, 927
计算分子量 14 kDa
观测分子量 14 kDa, 17 kDa
GenBank蛋白编号BC000897
基因名称 IFITM1
Gene ID (NCBI) 8519
RRIDAB_2919893
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13164
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

实验方案

Product Specific Protocols
IF protocol for CL594 IFITM1-Specific antibody CL594-60074Download protocol
Standard Protocols
Click here to view our Standard Protocols