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验证数据展示

  • IF Staining of rat brain using CL488-81728

    IF Staining of rat brain using CL488-81728

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 IBA1 antibody (CL488-81728, Clone: 4D5 ) at dilution of 1:200.

  • IF Staining of rat brain using CL488-81728

    IF Staining of rat brain using CL488-81728

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 IBA1 antibody (CL488-81728, Clone: 4D5 ) at dilution of 1:200.

  • FC experiment of THP-1 using CL488-81728

    FC experiment of THP-1 using CL488-81728

    1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human IBA1 (CL488-81728, Clone:4D5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated IBA1 Recombinant monoclonal antibody

IBA1 Uni-rAb® Recombinant Antibody for IF-P, FC (Intra)
Cat No. CL488-81728

产品说明书

CloneNo. 4D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

AIF1, 4D5, AIF 1, AIF-1, allograft inflammatory factor 1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inrat brain tissue
Positive FC (Intra) detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy IBA1 Ready-To-Use IHC Kit. IBA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81728-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81728 targets IBA1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1363

Product name: Recombinant human IBA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-147 aa of BC009474

Sequence: MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 allograft inflammatory factor 1
别名 AIF1, 4D5, AIF 1, AIF-1, allograft inflammatory factor 1
计算分子量 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC009474
基因名称 IBA1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
RRIDAB_3084505
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55008
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IBA1 antibody CL488-81728Download protocol
Standard Protocols
Click here to view our Standard Protocols
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