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验证数据展示

  • WB analysis using 81728-1-RR (same clone as 81728-1-PBS)

    WB analysis using 81728-1-RR (same clone as 81728-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human tonsil using 81728-1-RR (same clone as 81728-1-PBS)

    IHC staining of human tonsil using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil slide using 81728-1-RR (IBA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsil using 81728-1-RR (same clone as 81728-1-PBS)

    IHC staining of human tonsil using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

    FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.4 ug IBA1 Recombinant antibody (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

IBA1 Recombinant monoclonal antibody, PBS Only

IBA1 Uni-rAb® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA
Cat No. 81728-1-PBS

产品说明书

CloneNo. 4D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA

别名

AIF1, 4D5, AIF 1, AIF-1, allograft inflammatory factor 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IBA1 Ready-To-Use IHC Kit. IBA1 primary antibody included.

产品信息

81728-1-PBS targets IBA1 in WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IHC-Autostainer, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1363

Product name: Recombinant human IBA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-147 aa of BC009474

Sequence: MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 allograft inflammatory factor 1
别名 AIF1, 4D5, AIF 1, AIF-1, allograft inflammatory factor 1
计算分子量 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC009474
基因名称 IBA1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55008
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

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