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验证数据展示

  • IHC staining of human cerebellum using 66553-1-Ig

    IHC staining of human cerebellum using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included
  • IHC staining of human cerebellum using 66553-1-Ig

    IHC staining of human cerebellum using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included
  • IF Staining of SH-SY5Y using 66553-1-Ig

    IF Staining of SH-SY5Y using 66553-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Huntingtin antibody (66553-1-Ig, Clone: 2F6C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of rat cerebellum using 66553-1-Ig

    IF Staining of rat cerebellum using 66553-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Huntingtin antibody (66553-1-Ig, Clone: 2F6C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), FUS/TLS antibody (11570-1-AP, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Huntingtin Monoclonal antibody

Huntingtin Monoclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 66553-1-Ig

产品说明书

CloneNo. 2F6C9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IHC, IF/ICC, IF-P, ELISA

别名

HTT, 2F6C9, HD, HD protein, Huntingtin, myristoylated N-terminal fragment

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat cerebellum tissue
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy HTT Ready-To-Use IHC Kit. HTT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66553-1-Ig targets Huntingtin in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Huntingtin fusion protein Ag25922 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 huntingtin
别名 HTT, 2F6C9, HD, HD protein, Huntingtin, myristoylated N-terminal fragment
计算分子量 348 kDa
GenBank蛋白编号NM_002111
基因名称 Huntingtin
Gene ID (NCBI) 3064
RRIDAB_2881915
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Huntingtin antibody 66553-1-IgDownload protocol
IF protocol for Huntingtin antibody 66553-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols