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验证数据展示

  • IHC staining of human cerebellum using 66553-1-Ig (same clone as 66553-1-PBS)

    IHC staining of human cerebellum using 66553-1-Ig (same clone as 66553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66553-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HTT antibody included
  • IHC staining of human cerebellum using 66553-1-Ig (same clone as 66553-1-PBS)

    IHC staining of human cerebellum using 66553-1-Ig (same clone as 66553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66553-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HTT antibody included
  • IF Staining of SH-SY5Y using 66553-1-Ig (same clone as 66553-1-PBS)

    IF Staining of SH-SY5Y using 66553-1-Ig (same clone as 66553-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Huntingtin antibody (66553-1-Ig, Clone: 2F6C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66553-1-PBS in a different storage buffer formulation.

  • IF Staining of rat cerebellum using 66553-1-Ig (same clone as 66553-1-PBS)

    IF Staining of rat cerebellum using 66553-1-Ig (same clone as 66553-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Huntingtin antibody (66553-1-Ig, Clone: 2F6C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), FUS/TLS antibody (11570-1-AP, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66553-1-PBS in a different storage buffer formulation.

Huntingtin Monoclonal antibody, PBS Only

Huntingtin Monoclonal Antibody for IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66553-1-PBS

产品说明书

CloneNo. 2F6C9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IHC, IF/ICC, IF-P, Indirect ELISA

别名

HTT, 2F6C9, HD, HD protein, Huntingtin, myristoylated N-terminal fragment

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HTT Ready-To-Use IHC Kit. HTT primary antibody included.

产品信息

66553-1-PBS targets Huntingtin in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25922

Product name: Recombinant human Huntingtin protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 2845-3000 aa of NM_002111

Sequence: LDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 huntingtin
别名 HTT, 2F6C9, HD, HD protein, Huntingtin, myristoylated N-terminal fragment
计算分子量 348 kDa
GenBank蛋白编号NM_002111
基因名称 Huntingtin
Gene ID (NCBI) 3064
RRIDAB_2881915
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP42858
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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