验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98758-3-RR targets IgA1 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg9190 Product name: Recombinant human IgA1-IgK protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Sequence: \ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant alpha 1 |
| 别名 | Human IgA, Human IgA Heavy Chain, IGHA1, IgA, Igh2 |
| 基因名称 | Human IgA Heavy Chain |
| Gene ID (NCBI) | 3493 |
| RRID | AB_3746325 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
Immunoglobulins (Ig), also known as antibodies, are glycoproteins produced by B cells and plasma cells. There are 5 main classes of mammalian immunoglobulin heavy chains. Each class defines the IgM, IgG, IgA, IgD, and IgE isotypes (PMID: 20176268). IgA is the most prevalent antibody class at mucosal sites with an important role in mucosal defense. There are 2 subclasses of IgA, IgA1 and IgA2, which differ over a stretch of 18 amino acids in their hinge region, with the hinge of IgA1 being 13 amino acids longer than that of IgA2 (PMID: 21691145). This antibody specifically recognizes human IgA1 and does not cross-react with IgA2.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for IgA1 antibody 98758-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
