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验证数据展示

  • WB analysis using 83307-3-RR

    WB analysis using 83307-3-RR

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 83307-3-RR (Histone H2A.X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney(renal cell) Cancer using 83307-3-RR

    IHC staining of human kidney(renal cell) Cancer using 83307-3-RR

    Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with H2AX antibody included

Histone H2A.X Recombinant antibody

Histone H2A.X Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83307-3-RR

产品说明书

CloneNo. 250032F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

H2AFX, H2A histone family, member X, H2A.X, H2AX, Histone H2A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman kidney(renal cell) Cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy H2AX Ready-To-Use IHC Kit. H2AX primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83307-3-RR targets Histone H2A.X in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Histone H2A.X fusion protein Ag1305 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 H2A histone family, member X
别名 H2AFX, H2A histone family, member X, H2A.X, H2AX, Histone H2A
计算分子量 15 kDa
观测分子量 15-18 kDa, 22 kDa
GenBank蛋白编号BC013416
基因名称 Histone H2A.X
Gene ID (NCBI) 3014
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16104
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.

实验方案

Product Specific Protocols
WB protocol for Histone H2A.X antibody 83307-3-RRDownload protocol
IHC protocol for Histone H2A.X antibody 83307-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols