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验证数据展示

  • IF Staining of MCF-7 using CL488-83993-4

    IF Staining of MCF-7 using CL488-83993-4

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Histone H1.0 antibody (CL488-83993-4, Clone: 241147F6 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated Histone H1.0 Recombinant antibody

Histone H1.0 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83993-4

产品说明书

CloneNo. 241147F6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

H1F0, 241147F6, H1 histone family, member 0, H10, H1-0

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Histone H1.0 Ready-To-Use IHC Kit. Histone H1.0 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83993-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83993-4 targets Histone H1.0 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Histone H1.0 fusion protein Ag9982 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 H1 histone family, member 0
别名 H1F0, 241147F6, H1 histone family, member 0, H10, H1-0
计算分子量 21 kDa
GenBank蛋白编号BC000145
基因名称 Histone H1.0
Gene ID (NCBI) 3005
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07305
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Histone H1.0 antibody CL488-83993-4Download protocol
Standard Protocols
Click here to view our Standard Protocols