验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells, rat brain tissue, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Planning an IHC experiment? We recommend our IHCeasy HTATSF1 Ready-To-Use IHC Kit. HTATSF1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20805-1-AP targets HTATSF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | HTATSF1 fusion protein Ag14744 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HIV-1 Tat specific factor 1 |
| 别名 | Tat-SF1, TAT SF1, HIV Tat-specific factor 1, 17S U2 SnRNP complex component HTATSF1 |
| 计算分子量 | 755 aa, 86 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | BC009896 |
| 基因名称 | HTATSF1 |
| Gene ID (NCBI) | 27336 |
| RRID | AB_10695767 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43719 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HTATSF1 antibody 20805-1-AP | Download protocol |
| IHC protocol for HTATSF1 antibody 20805-1-AP | Download protocol |
| IF protocol for HTATSF1 antibody 20805-1-AP | Download protocol |
| IP protocol for HTATSF1 antibody 20805-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.
| ||
Nucleic Acids Res Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II. | ||
Cell Death Discov Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.
| ||
Cell Rep ArreSTick motif controls β-arrestin-binding stability and extends phosphorylation-dependent β-arrestin interactions to non-receptor proteins |
