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验证数据展示

  • FC experiment of HeLa using CL488-68059

    FC experiment of HeLa using CL488-68059

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HSPB11 (CL488-68059, Clone:2D11B3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated HSPB11 Monoclonal antibody

HSPB11 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68059

产品说明书

CloneNo. 2D11B3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

Heat shock protein beta-11, Heat shock protein beta 11, FAP232, CFAP232, C1orf41

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68059-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68059 targets HSPB11 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 HSPB11 fusion protein Ag8804 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock protein family B (small), member 11
别名 Heat shock protein beta-11, Heat shock protein beta 11, FAP232, CFAP232, C1orf41
计算分子量 144 aa, 16 kDa
观测分子量 20 kDa
GenBank蛋白编号BC005245
基因名称 HSPB11
Gene ID (NCBI) 51668
RRIDAB_2923875
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y547
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 HSPB11 antibody CL488-68059Download protocol
Standard Protocols
Click here to view our Standard Protocols