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验证数据展示

  • WB analysis using 68059-1-Ig

    WB analysis using 68059-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68059-1-Ig (HSPB11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68059-1-Ig

    WB analysis of HeLa using 68059-1-Ig

    WB result of HSPB11 antibody (68059-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPB11 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

HSPB11 Monoclonal antibody

HSPB11 Monoclonal Antibody for WB, ELISA
Cat No. 68059-1-Ig

产品说明书

CloneNo. 2D11B3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, ELISA

别名

Heat shock protein beta-11, Heat shock protein beta 11, FAP232, CFAP232, C1orf41

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCCIT cells, HeLa cells, LNCaP cells, HEK-293 cells, T-47D cells, Jurkat cells, K-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68059-1-Ig targets HSPB11 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HSPB11 fusion protein Ag8804 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock protein family B (small), member 11
别名 Heat shock protein beta-11, Heat shock protein beta 11, FAP232, CFAP232, C1orf41
计算分子量 144 aa, 16 kDa
观测分子量 20 kDa
GenBank蛋白编号BC005245
基因名称 HSPB11
Gene ID (NCBI) 51668
RRIDAB_2918800
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y547
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.

实验方案

Product Specific Protocols
WB protocol for HSPB11 antibody 68059-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols