验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HSPA1L Ready-To-Use IHC Kit. HSPA1L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13970-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13970 targets HSPA1L in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | HSPA1L fusion protein Ag4995 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heat shock 70kDa protein 1-like |
| 别名 | Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t |
| 计算分子量 | 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC034483 |
| 基因名称 | HSPA1L |
| Gene ID (NCBI) | 3305 |
| RRID | AB_3672581 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P34931 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognized the endogenous HSPA1L protein in multiple cell lines. (21730052)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HSPA1L antibody CL488-13970 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |