验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, MCF-7 cells, HeLa cells, HepG2 cells, human saliva tissue, rat testis tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human endometrial cancer tissue, human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HSPA1L Ready-To-Use IHC Kit. HSPA1L primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13970-1-AP targets HSPA1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, bovine |
| 免疫原 | HSPA1L fusion protein Ag4995 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heat shock 70kDa protein 1-like |
| 别名 | HSP70-Hom, HSP70 HOM, HSP70 1L, Heat shock protein family A member 1L, Heat shock 70 kDa protein 1-like |
| 计算分子量 | 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC034483 |
| 基因名称 | HSPA1L |
| Gene ID (NCBI) | 3305 |
| RRID | AB_2119675 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P34931 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognized the endogenous HSPA1L protein in multiple cell lines. (21730052)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA1L antibody 13970-1-AP | Download protocol |
| IHC protocol for HSPA1L antibody 13970-1-AP | Download protocol |
| IF protocol for HSPA1L antibody 13970-1-AP | Download protocol |
| IP protocol for HSPA1L antibody 13970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Endocrinol (Lausanne) Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring. | ||
Virol J Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9. | ||
PeerJ The heat shock protein family gene Hspa1l in male mice is dispensable for fertility.
| ||
Neurobiol Dis SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function | ||
J Cachexia Sarcopenia Muscle The regulation of MFG-E8 on the mitophagy in diabetic sarcopenia via the HSPA1L-Parkin pathway and the effect of D-pinitol | ||
Biomolecules Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model |
