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验证数据展示

  • WB analysis using 83689-3-RR (same clone as 83689-3-PBS)

    WB analysis using 83689-3-RR (same clone as 83689-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83689-3-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83689-3-RR (same clone as 83689-3-PBS)

    WB analysis of HeLa using 83689-3-RR (same clone as 83689-3-PBS)

    WB result of HSP47/SERPINH1 antibody (83689-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47/SERPINH1 transfected HeLa cells. This data was developed using the same antibody clone with 83689-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83689-3-RR (same clone as 83689-3-PBS)

    IHC staining of human ovarian cancer using 83689-3-RR (same clone as 83689-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83689-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP47 antibody included
  • FC experiment of HepG2 using 83689-3-RR (same clone as 83689-3-PBS)

    FC experiment of HepG2 using 83689-3-RR (same clone as 83689-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HSP47/SERPINH1 Recombinant antibody (83689-3-RR, Clone:240752F6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83689-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP00677-2

    Sandwich ELISA sample test of MP00677-2

    HepG2, Hela and MCF-7 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Aliquots of the cell culture supernates were removed. Cells were lysed and assayed for human HSP47/SERPINH1. Human HSP47/SERPINH1 in HepG2 cell lysate measured 17.9 ng/mL. Human HSP47/SERPINH1 in Hela cell lysate measured 56.9 ng/mL. Human HSP47/SERPINH1 in MCF-7 cell lysate measured 35.1 ng/mL.

  • Sandwich ELISA standard curve of MP00677-2

    Sandwich ELISA standard curve of MP00677-2

    Sandwich ELISA standard curve of MP00677-2, Human HSP47/SERPINH1 Recombinant Matched Antibody Pair - PBS only. 83689-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1201. 83689-3-PBS was HRP conjugated as the detection antibody. Range: 0.23-15 ng/mL

  • Affinity and Kinetic Characterization of 83689-3-RR

    Affinity and Kinetic Characterization of 83689-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83689-3-RR against Human HSP47/SERPINH1 were performed. The affinity constant is 12.9 nM.

  • Featured Product
  • KD/KO验证

HSP47/SERPINH1 Recombinant monoclonal antibody, PBS Only (Detector)

HSP47/SERPINH1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA
Cat No. 83689-3-PBS

产品说明书

CloneNo. 240752F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

别名

HSP47, SERPINH1, 240752F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.

产品信息

83689-3-PBS targets HSP47/SERPINH1 as part of a matched antibody pair:

MP00677-2: 83689-4-PBS capture and 83689-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg1201

Product name: Recombinant Human HSP47/SERPINH1 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 19-418 aa of NM_001235.5

Sequence: AEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
别名 HSP47, SERPINH1, 240752F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3
计算分子量46kDa
观测分子量 46 kDa
GenBank蛋白编号NM_001235.5
基因名称 HSP47/SERPINH1
Gene ID (NCBI) 871
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP50454
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

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