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验证数据展示

  • WB analysis using 83689-3-RR

    WB analysis using 83689-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 83689-3-RR

    WB analysis of HeLa using 83689-3-RR

    WB result of HSP47/SERPINH1 antibody (83689-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47/SERPINH1 transfected HeLa cells.

  • IHC staining of human ovarian cancer using 83689-3-RR

    IHC staining of human ovarian cancer using 83689-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • FC experiment of HepG2 using 83689-3-RR

    FC experiment of HepG2 using 83689-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HSP47/SERPINH1 Recombinant antibody (83689-3-RR, Clone:240752F6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83689-3-RR

    Affinity and Kinetic Characterization of 83689-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83689-3-RR against Human HSP47/SERPINH1 were performed. The affinity constant is 12.9 nM.

  • Featured Product
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HSP47/SERPINH1 Recombinant monoclonal antibody

HSP47/SERPINH1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83689-3-RR

产品说明书

CloneNo. 240752F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

HSP47, SERPINH1, 240752F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HT-1080 cells, SK-N-SH cells, NIH/3T3 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83689-3-RR targets HSP47/SERPINH1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg1201

Product name: Recombinant Human HSP47/SERPINH1 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 19-418 aa of NM_001235.5

Sequence: AEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
别名 HSP47, SERPINH1, 240752F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3
计算分子量46kDa
观测分子量 46 kDa
GenBank蛋白编号NM_001235.5
基因名称 HSP47/SERPINH1
Gene ID (NCBI) 871
RRIDAB_3671291
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP50454
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

实验方案

Product Specific Protocols
IHC protocol for HSP47/SERPINH1 antibody 83689-3-RRDownload protocol
WB protocol for HSP47/SERPINH1 antibody 83689-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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